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the spatial resolution of the synapses in the test volume ranges from 2.1 to 2.8nm. from the electron microscopy images, it is not possible to estimate the precision of the volume-filling synapses, although the images have been recorded at a resolution of 2nm/pixel. the volume filling synapses are of course a larger sample of synapses. however, we expect that the contrast produced by these synapses is similar to other synapses, as the latter are visible in the close vicinity. in fig. 2, we show a closer look at the superposition of the synapses and selected background pixels. the spatial distances of the synapses are much larger than the dimensions of the nuclei and small vesicles, so that they are well separated from nuclei and other organelles.
the electron microscopy images are part of the data deposition, in the folder data/test_synapse_objects. each image has a size of 72x72x18 pixels, and the full dataset includes 13 volumes. the spatial resolution of the synapse images is 2.4nm per pixel, or 24nm for the whole volume. the first volume of the dataset includes the synapse candidate objects detected by the algorithm, whereas the other volumes include the final list of synapse candidates after pixel classification and object segmentation. the synapse candidates are in tiff format, the segmented synapse objects are in png format and the images are stored in pdf format. the naming convention for the synapse candidates is synapse_candidate_00
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